Later hosts Brazil will play Croatia in the first game of the tournament, which will be watched by a crowd of more than 60,000 people, including 12 foreign heads of state.
The ceremony began with children jumping up and down on trampolines and people dressed as trees walking around a huge globe in the centre of the pitch.
They were followed by dancers performing a variety of routines, some solo and others together.
JLo then appeared along with Pitbull and Ms Leitte.
The match, which kicks off at 9pm, comes after violent clashes between police and demonstrators who tried to block a key road leading to the stadium.
Officers fired tear gas and stun grenades to disperse around 300 protesters angry about the billions of dollars spent on the event.
Two goals from Neymar and a late Oscar strike inspired Brazil to a 3-1 comeback victory over Croatia in the opening game of the 2014 World Cup.
The host nation fell behind to an early own goal from Marcelo, before Neymar was perhaps fortunate to be shown just a yellow card after catching Luka Modric in the throat with a stray arm.
Soon after, the 22-year-old levelled with a fine 25-yard effort on his left foot, before converting a controversial second half penalty after Dejan Lovren was adjudged to have brought down Fred.
And as the game entered injury-time, man-of-the-match Oscar sped forward and toe-poked home an effort from the edge of the box to give Brazil breathing space and, most importantly, three points.
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